Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,494,543 T→C G24G (GGA→GGG)  yfdE ← predicted CoA‑transferase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,494,5430TC100.0% 269.8 / NA 94G24G (GGA→GGG) yfdEpredicted CoA‑transferase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/94);  total (0/94)

TACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGA  >  W3110S.gb/2494518‑2494553
                         |          
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:491677/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:643316/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:621571/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:615898/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:600036/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:58270/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:58030/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:574794/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:562619/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:546708/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:522677/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:50550/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:650432/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:490579/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:44172/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:428633/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:425962/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:358367/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:347174/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:343122/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:341887/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:340760/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:29952/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1041464/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:811549/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:970574/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:960477/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:947890/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:91988/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:916819/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:912010/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:8953/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:885248/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:877899/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:849620/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:815542/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:289074/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:778718/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:774471/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:770639/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:763898/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:746081/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:712080/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:705330/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:684766/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:671250/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:656213/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1226844/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1340757/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1325385/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1317365/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1310679/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1310115/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1293340/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1288765/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1287586/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1286244/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1279446/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1250936/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1356681/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1205228/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1202329/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1199487/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1173818/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1157635/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1143938/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1107145/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1080708/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1074965/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1068939/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:104294/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:147161/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:26496/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:244002/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:220536/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:200700/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:178794/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:177022/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:174496/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:156439/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:269409/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1445988/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1431475/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1398621/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1387994/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1382133/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1380721/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1363904/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1361538/36‑1 (MQ=255)
tACAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:1359325/36‑1 (MQ=255)
 aCAAAGAAGTTGAGTTCCGAAAGGCCCATTATGGa  <  1:17085/35‑1 (MQ=37)
 aCAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:459726/35‑1 (MQ=255)
 aCAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:168086/35‑1 (MQ=255)
 aCAAAGAAGTTGAGTTCCGAAAGGCCCATTAAGGa  <  1:139234/35‑1 (MQ=255)
                         |          
TACAAAGAAGTTGAGTTCCGAAAGGTCCATTAAGGA  >  W3110S.gb/2494518‑2494553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A