Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,504,241 |
Δ1 bp |
coding (125/1698 nt) |
ypdA → |
predicted sensory kinase in two‑component system with YpdB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,504,238 | 0 | C | . | 100.0%
| 32.3
/ NA
| 9 | coding (122/1698 nt) | ypdA | predicted sensory kinase in two‑component system with YpdB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (9/0); total (9/0) |
GTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCAT > W3110S.gb/2504206‑2504268
|
gTTTCGCGAACTGTTGCACAAGTCGTCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt > 1:722547/1‑62 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCa > 1:1178450/1‑61 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCa > 1:575415/1‑61 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt > 1:380600/1‑62 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt > 1:420767/1‑62 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt > 1:426802/1‑62 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt > 1:6160/1‑62 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt > 1:989152/1‑62 (MQ=255)
gTTTCGCGAACTGTTGCACAAGTCGGCGCACT‑CCCAAAAGAATTGCTCGCCGTTACCGCCAt > 1:993772/1‑62 (MQ=255)
|
GTTTCGCGAACTGTTGCACAAGTCGGCGCACTCCCCAAAAGAATTGCTCGCCGTTACCGCCAT > W3110S.gb/2504206‑2504268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
