Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,675,556 C→A A488S (GCT→TCT)  yphE ← fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,675,5560CA95.6% 114.8 / ‑1.9 45A488S (GCT→TCT) yphEfused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (0/43);  total (0/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTA  >  W3110S.gb/2675520‑2675588
                                    |                                
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:639633/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:19867/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:201916/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:233844/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:295335/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:301607/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:393840/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:395742/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:501213/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:616912/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:629597/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1092139/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:683122/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:683128/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:698419/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:7167/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:751484/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:765695/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:780337/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:79735/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:831164/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:15648/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1056611/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1109836/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1170065/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1189644/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1191780/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:120127/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1216186/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1247364/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1289548/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1299214/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1334058/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1357555/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1400340/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1450967/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1464953/62‑1 (MQ=255)
gaATGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:186767/62‑1 (MQ=255)
 aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAActtgccg         <  1:1113715/61‑1 (MQ=255)
 aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:617162/61‑1 (MQ=255)
 aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:1466444/61‑1 (MQ=255)
 aaTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:912380/61‑1 (MQ=255)
  aTGGCGGACATCAGCTCATCCACATTGACCGGAGAGTGAAACTCCTGCGAGAAcgtgccg         <  1:90468/60‑1 (MQ=255)
       ggACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGta  <  1:1246862/62‑1 (MQ=255)
       ggACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGta  <  1:434422/62‑1 (MQ=255)
                                    |                                
GAATGGCGGACATCAGCTCATCCACATTGACCGGAGCGTGAAACTCCTGCGAGAACGTGCCGTGCTGTA  >  W3110S.gb/2675520‑2675588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A