Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,804,902 C→T F79F (TTC→TTT)  proW → glycine betaine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,804,9020CT100.0% 250.3 / NA 89F79F (TTC→TTT) proWglycine betaine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/89);  total (0/89)

GCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCC  >  W3110S.gb/2804854‑2804915
                                                |             
gCTCGACAGTTGGTTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1139441/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:529661/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:569789/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:508318/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:503938/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:495242/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:464726/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:896065/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:434978/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:432818/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:432534/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:426469/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:420964/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:408264/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:403058/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:38072/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:361908/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:35934/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:344245/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:287354/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:246662/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:703313/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:836814/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:82498/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:819133/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:791724/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:741198/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:72607/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:725314/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:723273/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:717713/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:561948/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:645088/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:631517/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:62997/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:845335/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:852683/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:605921/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:602266/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:570787/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:233027/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1156946/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1326890/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1310813/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1309565/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1272908/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:124940/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1246049/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1208703/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:90552/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1158048/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1341479/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:917599/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1129994/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1123235/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:969997/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1082830/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1061407/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1056573/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1048971/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:10077/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:146170/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:229586/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:218408/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:204534/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:202344/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:19407/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:191858/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:179040/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:162589/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:155854/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1448055/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1435221/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1421553/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1403179/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1378348/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1373974/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1365387/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1363995/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTCTCGTCCCGTCtttc  <  1:1013237/62‑3 (MQ=255)
gCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTAGTTACCCATTTTCGTCCCGTCtttc  <  1:863221/62‑3 (MQ=255)
gCTCGACAGTTGGGGCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1111348/62‑3 (MQ=255)
           gggTCACTGAAGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:617817/51‑3 (MQ=255)
              tCACTGAAGGGATCGACTGGGTCGTTACCCACTTTCGTCCCGTCtttc  <  1:61635/48‑3 (MQ=38)
                    aaGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:870722/42‑3 (MQ=39)
                    aaGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:441126/42‑3 (MQ=39)
                    aaGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:972177/42‑3 (MQ=39)
                     aGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1179718/41‑3 (MQ=39)
                     aGGGATCGACTGGGTCGTTACCCATTTTCGTCCCGTCtttc  <  1:1135060/41‑3 (MQ=39)
                                                |             
GCTCGACAGTTGGGTCACTGAAGGGATCGACTGGGTCGTTACCCATTTCCGTCCCGTCTTCC  >  W3110S.gb/2804854‑2804915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A