Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,874,423 |
+GT |
coding (563/909 nt) |
cysD ← |
sulfate adenylyltransferase, subunit 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,874,421 | 1 | . | G | 100.0%
| 34.6
/ NA
| 14 | W189R (TGG→CGG) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (14/0); total (14/0) |
| * | W3110S.gb | 2,874,421 | 2 | . | T | 100.0%
| 32.9
/ NA
| 14 | W189R (TGG→AGG) | cysD | sulfate adenylyltransferase, subunit 2 |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (14/0); total (14/0) |
ATATCCTGCTCGGTCCA‑‑GTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATT > W3110S.gb/2874405‑2874464
||
atatCCTGCTCTGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:351630/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTaaat > 1:300028/1‑60 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:1035726/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:1045908/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:124011/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:152732/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:497384/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:589184/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:691994/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:913074/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAtt > 1:956110/1‑62 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt > 1:1256437/1‑61 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt > 1:181329/1‑61 (MQ=255)
atatCCTGCTCGGTCCAGTGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt > 1:675393/1‑61 (MQ=255)
||
ATATCCTGCTCGGTCCA‑‑GTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATT > W3110S.gb/2874405‑2874464
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
