Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,098,749 C→T Q212Q (CAG→CAA)  ansB ← periplasmic L‑asparaginase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,098,7490CT100.0% 568.0 / NA 203Q212Q (CAG→CAA) ansBperiplasmic L‑asparaginase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (203/0);  total (203/0)

Only 100 of 203 total aligned reads displayed.

TGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAG  >  W3110S.gb/3098729‑3098801
                    |                                                    
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGtt                                  >  1:729337/1‑41 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:617457/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:139774/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1445315/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1465496/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:155196/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:18538/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:195256/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:233713/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:270245/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:359691/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:417254/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:424771/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:4496/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:520902/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:573246/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:608247/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:299860/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:645637/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:675779/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:684957/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:693377/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:697906/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:712525/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:788094/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:796488/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:810511/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:881050/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:948353/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:976243/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:98129/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1166831/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1148923/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1247790/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1102699/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1151431/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1085555/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:10654/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1218225/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1282206/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1035845/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1310580/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1346178/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1020794/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1028589/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1202332/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:1297597/1‑42 (MQ=255)
tGCTTACGTGCCGGGGTACGTTGGTAGTCAATCTTACAGTtg                                 >  1:1291364/1‑42 (MQ=255)
tGCTTACGAGCCGGGGTACGTTGGTAGTCAATCTTACCGTtg                                 >  1:743575/1‑42 (MQ=255)
           cGGTGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:89944/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAAccc                     >  1:66535/1‑43 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAAc                       >  1:565199/1‑41 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACa   >  1:1037947/1‑61 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACa   >  1:1356415/1‑61 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACa   >  1:1222069/1‑61 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACa   >  1:412058/1‑61 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:747150/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:679770/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:630777/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1163560/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:643743/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1122144/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1100369/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:544632/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:791772/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1043693/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:82713/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:835794/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:840915/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:932529/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:934221/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1007769/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:589924/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1378460/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1337112/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1454013/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1456068/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1327540/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:17366/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:189702/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:19455/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:218975/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:258772/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1271490/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:281545/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:295862/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:301810/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1261383/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:37444/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:376419/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:383500/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1240740/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:456429/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:478480/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:487092/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:505448/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:1213545/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:58525/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAg  >  1:622276/1‑62 (MQ=255)
           cGGGGTACGTTGGTAGTCAATCTTACCGTTGTGAATGTAACCAAGAGGACCGTAGTTAACAg  >  1:1003223/1‑62 (MQ=255)
                    |                                                    
TGCTTACGTGCCGGGGTACGCTGGTAGTCAATCTTACCGTTGTGAATGTAACCCAGAGGACCGTAGTTAACAG  >  W3110S.gb/3098729‑3098801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A