Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,355,794 |
T→C |
R436R (CGT→CGC) |
gltB → |
glutamate synthase, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,355,794 | 0 | T | C | 100.0%
| 386.8
/ NA
| 134 | R436R (CGT→CGC) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/134); total (0/134) |
Only 100 of 134 total aligned reads displayed.
GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACC > W3110S.gb/3355755‑3355815
|
gCCAGGCGAACTGATGGTGATCTACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:571940/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:453594/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:546990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:543352/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:528153/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:514269/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:506347/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:495806/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:494253/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:484199/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:465617/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:465084/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:46391/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:55040/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:445254/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:416212/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:395049/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:383866/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:381841/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:33002/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:30914/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:29733/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1011408/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:263836/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:262891/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:800483/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:991222/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:988187/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:976123/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:965916/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:964171/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:953511/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:896600/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:878972/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:869129/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:863124/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:804285/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:27591/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:757029/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:745160/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:702959/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:700990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:654418/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:640839/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:634371/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:629131/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:622793/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:606421/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1215707/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1291106/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1289459/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1256692/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:245894/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1253400/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1245692/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1242443/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1234076/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1229125/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1076762/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1223769/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1317863/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1211157/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1101908/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1133776/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1185744/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:118184/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1172821/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1169875/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1167990/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1155492/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1135441/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1447911/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:242730/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:238817/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:220237/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:215117/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:197092/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1063743/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1459998/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1455878/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1455150/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1444408/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1444348/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1404176/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:14001/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1397914/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:107666/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1377596/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1369474/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1349793/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1349159/61‑1 (MQ=255)
gCCAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCCCATTCTACACTCGGCAGAAAcc < 1:364905/61‑1 (MQ=255)
ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:56641/60‑1 (MQ=255)
ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1196712/60‑1 (MQ=255)
ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1382870/60‑1 (MQ=255)
ccAGGCGAACTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:338231/60‑1 (MQ=255)
cTGATGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:63718/51‑1 (MQ=255)
tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:924871/47‑1 (MQ=255)
tGGTGATCGACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:119350/47‑1 (MQ=255)
gACACTCGCAGTGGGCGCATTCTACACTCGGCAGAAAcc < 1:1063579/39‑1 (MQ=37)
|
GCCAGGCGAACTGATGGTTATCGACACCCGCAGTGGGCGTATTCTGCACTCGGCAGAAACC > W3110S.gb/3355755‑3355815
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
