Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,429,766 |
+A |
coding (618/1590 nt) |
purH → |
fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,429,763 | 1 | . | A | 100.0%
| 18.7
/ NA
| 9 | S205R (AGC→AGA) | purH | fused IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/9); total (0/9) |
GCATGGTTCCGGCTTACCACGGTGAAAGC‑AAAGAAGCCGCCGGTCGCTTCCCACGCACGCT > W3110S.gb/3429735‑3429795
|
gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1013963/62‑1 (MQ=255)
gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1425907/62‑1 (MQ=255)
gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1440393/62‑1 (MQ=255)
gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:25206/62‑1 (MQ=255)
gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:858460/62‑1 (MQ=255)
gcaTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:967629/62‑1 (MQ=255)
caTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1212924/61‑1 (MQ=255)
caTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:1363040/61‑1 (MQ=255)
caTGGTTCCGGCTTACCACGGTGAAAGCAAAAGAAGCCGCCGGTCGCTTCCCACGCACGct < 1:429546/61‑1 (MQ=255)
|
GCATGGTTCCGGCTTACCACGGTGAAAGC‑AAAGAAGCCGCCGGTCGCTTCCCACGCACGCT > W3110S.gb/3429735‑3429795
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
