Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,789,435 |
Δ1 bp |
coding (111/291 nt) |
ilvN → |
acetolactate synthase I, small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,789,434 | 0 | A | . | 100.0%
| 80.0
/ NA
| 20 | coding (110/291 nt) | ilvN | acetolactate synthase I, small subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (20/0); total (20/0) |
ACGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTC > W3110S.gb/3789395‑3789457
|
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGAtt > 1:194544/1‑61 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:1058825/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:963538/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:93564/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:930480/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:824862/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:78118/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:734889/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:630571/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:589040/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:567950/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:197803/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:194123/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:192571/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:137393/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:1372508/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:1359670/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:1301999/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:1056031/1‑62 (MQ=255)
aCGTTTGTGGCCTTTTTGCCC‑CCGCGCTTTTAACGTTG‑AGGCATTCTTTGTCTGCCGATTc > 1:414517/1‑61 (MQ=255)
|
ACGTTTGTGGCCTTTTTGCCCGCCGCGCTTTTAACGTTGAAGGCATTCTTTGTCTGCCGATTC > W3110S.gb/3789395‑3789457
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
