Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,069,946 T→C D283D (GAT→GAC)  glgX → glycogen debranching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,069,9460TC97.1% 273.9 / ‑3.1 102D283D (GAT→GAC) glgXglycogen debranching enzyme
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base C (99/0);  total (102/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 102 total aligned reads displayed.

CCTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCG  >  W3110S.gb/4069910‑4069998
                                    |                                                    
ccTCGGCGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:309566/1‑60 (MQ=255)
ccTCGCCGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:460950/1‑60 (MQ=255)
ccTCGCCGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:741060/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTGTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:609864/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTGGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:173337/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCtatta                                        >  1:684469/1‑51 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTg                                     >  1:610456/1‑54 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTg                                     >  1:1439922/1‑54 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGa                                   >  1:1093281/1‑56 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATaa                                >  1:1156351/1‑59 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATa                                 >  1:466504/1‑58 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:378465/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:476668/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:411172/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:412354/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:425953/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:435307/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:594766/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:376128/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:368021/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:358083/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:307341/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:289594/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:288867/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:286969/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:273441/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:272319/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:244322/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:243523/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:812865/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:948487/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:944745/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:932713/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:922328/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:909628/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:866494/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:864560/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:84452/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:836554/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:828015/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:572260/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:77886/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:754889/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:741022/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:684984/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:670230/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:664409/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:625837/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:233218/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:594707/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:578087/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1168524/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1299613/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1296647/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1289061/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1275947/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:126904/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1240357/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1231913/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1231042/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1222376/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1196175/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1185590/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1336671/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1158679/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1155588/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1103043/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1091486/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1079343/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1078384/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1066790/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1066527/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1034889/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1032611/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1028645/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1391788/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:191914/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:190892/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:18259/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1740/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:16236/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:161799/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:15938/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:149842/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:148687/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1425365/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:102403/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1374739/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1367564/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1364652/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1355452/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1351705/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1351018/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1349806/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:1341687/1‑60 (MQ=255)
ccTCGACGGCCCGTTATTCTAGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:562354/1‑60 (MQ=255)
 cTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag                               >  1:896200/1‑59 (MQ=255)
                           tGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCg  >  1:1396958/1‑62 (MQ=255)
                           tGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCg  >  1:1350763/1‑62 (MQ=255)
                           tGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCg  >  1:570491/1‑62 (MQ=255)
                                    |                                                    
CCTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCG  >  W3110S.gb/4069910‑4069998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A