Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,152,194 |
T→C |
L478L (CTA→CTG) |
yhfK ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,152,194 | 0 | T | C | 80.8%
| 218.8
/ 36.2
| 125 | L478L (CTA→CTG) | yhfK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/24); new base C (101/0); total (101/24) |
| Fisher's exact test for biased strand distribution p-value = 3.11e-26 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 125 total aligned reads displayed.
CCAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTGCAGGGTATACACTGCGGTAATAGTAA > W3110S.gb/4152138‑4152225
|
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:850902/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:757181/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:197593/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:593712/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:219916/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1191100/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1156563/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1065903/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:519560/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1003321/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:682225/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:488592/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:437918/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:551068/62‑1 (MQ=255)
ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGATCAACCACAATAGTTg < 1:791206/62‑1 (MQ=255)
cAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1329881/61‑1 (MQ=255)
cAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1350378/61‑1 (MQ=255)
aataatGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:182297/60‑1 (MQ=255)
cGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1406775/49‑1 (MQ=255)
atcGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1068393/3‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATGGTaa > 1:519882/2‑47 (MQ=25)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:601070/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:535656/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:627368/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:62990/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:588014/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:539896/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:538484/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:724016/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:534650/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:51977/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:504390/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:480504/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:473363/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:766421/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:97246/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:968900/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:927051/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:909885/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:874633/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:858594/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:858321/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:829713/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:808793/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:768454/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:665075/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:765795/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:760114/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:752311/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:749752/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:741651/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:740427/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:337918/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:711662/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:706982/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:670021/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1132465/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1413712/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1392356/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1388462/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1386100/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:132889/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1326133/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1317911/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1316996/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1304545/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1277243/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:142888/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1111360/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1076211/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1072867/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1062453/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1053384/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1046580/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1044391/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1036585/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:102673/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1024041/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:225664/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:444765/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:395000/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:34443/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:33346/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:316096/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:300682/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:282073/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:265677/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:457659/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:20449/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:204049/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:185768/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:184665/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:166278/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:165486/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:162565/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:162246/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1441654/2‑47 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTa > 1:161102/2‑46 (MQ=37)
accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGCTAATAGTaa > 1:494297/2‑47 (MQ=25)
accGTTCAGACACAACAGTTGCAGGGTATACACTGCGGTaa > 1:276769/2‑41 (MQ=25)
|
CCAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTGCAGGGTATACACTGCGGTAATAGTAA > W3110S.gb/4152138‑4152225
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
