Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,171,162 |
T→G |
S200A (TCA→GCA) |
chiA → |
periplasmic endochitinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,171,162 | 0 | T | G | 100.0%
| 225.7
/ NA
| 79 | S200A (TCA→GCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (79/0); total (79/0) |
CAGCCTTCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTCAGCCAGC > W3110S.gb/4171156‑4171217
|
caGCCTGCACCGGATGTGAAACCGGCCCCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1144958/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:492914/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:693017/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:681167/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:638301/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:625885/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:60745/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:57415/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:547080/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:511924/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:501490/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:723934/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:484709/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:478190/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:470089/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:460702/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:443613/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:438480/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:434279/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:430721/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:878662/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:994861/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:974713/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:930972/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:923033/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:907546/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:900895/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:898054/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:889905/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:887331/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:718872/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:859846/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:840352/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:837818/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:837372/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:801011/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:786413/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:77/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:41958/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1184998/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1012179/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1271061/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1266410/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1263517/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1236633/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1217300/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1209614/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1209466/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1187370/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1271093/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1179441/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1176803/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1167088/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:116402/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:115317/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1125225/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1080197/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1076831/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1436003/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:333676/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:327988/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:320869/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:319868/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:301404/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:30127/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:217176/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:168296/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:154699/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1294466/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1434230/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1411265/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1407772/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1392734/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1368318/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1337917/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc > 1:1298677/1‑62 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccag > 1:834140/1‑61 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccag > 1:81813/1‑61 (MQ=255)
caGCCTGCACCGGATGTGAAACCGGCACCCGACGTTAAACCGGCTCCTCATCTTcagccagc > 1:175340/1‑62 (MQ=255)
|
CAGCCTTCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTCAGCCAGC > W3110S.gb/4171156‑4171217
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
