Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,260,515 |
A→T |
D96V (GAT→GTT) |
dgkA → |
diacylglycerol kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,260,515 | 0 | A | T | 100.0%
| 129.0
/ NA
| 48 | D96V (GAT→GTT) | dgkA | diacylglycerol kinase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/48); total (0/48) |
CCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCG > W3110S.gb/4260489‑4260550
|
gcATGAGCTTTCCGGACGCGCAAAAGTTATGTGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1342324/61‑1 (MQ=39)
gcATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:274897/61‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:812244/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:518288/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:578345/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:636710/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:667703/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:70243/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:718052/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:718145/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:744041/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:748676/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:787781/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:808454/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:513126/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:826765/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:831614/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:880058/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:906881/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:939887/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:949235/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:965181/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:981431/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:996427/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:998051/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:175320/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1071322/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1128570/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:122553/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1250554/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1251707/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:125362/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1382546/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1448684/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1451979/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:14523/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:1025396/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:223299/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:228033/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:266975/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:33139/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:338972/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:342541/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:400263/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:400542/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:474213/62‑1 (MQ=255)
ccATGAGCTTTCCGGACGCGCAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:489173/62‑1 (MQ=255)
gcgcAAAAGTTATGGGATCCGCTGCGGTGCTGATTGCCATTAtcg < 1:851558/45‑1 (MQ=255)
|
CCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCG > W3110S.gb/4260489‑4260550
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
