Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I2 R1 721 0.0 1472758 54.8% 807071 56.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,489,902 T→C Q244Q (CAA→CAG)  pepA ← aminopeptidase A, a cyteinylglycinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,489,9020TC100.0% 203.3 / NA 72Q244Q (CAA→CAG) pepAaminopeptidase A, a cyteinylglycinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (72/0);  total (72/0)

CATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCT  >  W3110S.gb/4489887‑4489947
               |                                             
cATCAGCGATTCGTTCTGCGAACCCTGCCCGACCGCCAGAt                      >  1:140446/1‑41 (MQ=38)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAg                        >  1:1099716/1‑39 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGaa                 >  1:1247517/1‑46 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCTTCCCCAGctct  >  1:317335/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:660604/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:742497/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:660018/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:645910/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:609892/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:558517/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:534578/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:516078/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:512845/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:481263/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:43594/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:41956/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:410251/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:407983/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:37264/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:356432/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:827757/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:996446/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:996409/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:981391/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:957980/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:953285/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:925995/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:893301/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:845515/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:733748/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:820473/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:813756/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:805777/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:797967/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:77855/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:766903/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:75721/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:277203/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1117766/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1267864/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1249106/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1207860/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1168830/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1150630/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1136318/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:112898/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1127813/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1297499/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1099590/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:106033/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1044459/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1038029/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1028576/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1023878/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1007376/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1467642/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:100440/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:268533/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:249232/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:196209/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:19097/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:188302/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1468414/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:318983/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1411422/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1371761/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1361156/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1343666/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1324409/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1302913/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1300673/1‑61 (MQ=255)
cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctc   >  1:148281/1‑60 (MQ=255)
               |                                             
CATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCT  >  W3110S.gb/4489887‑4489947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A