Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I2 R1
|
721 |
0.0 |
1472758 |
54.8% |
807071 |
56.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,619,282 |
2 bp→TA |
intergenic (+340/+79) |
yjjV → / ← yjjW |
predicted DNase/predicted pyruvate formate lyase activating enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,619,282 | 0 | C | T | 100.0%
| 53.9
/ NA
| 35 | intergenic (+340/+80) | yjjV/yjjW | predicted DNase/predicted pyruvate formate lyase activating enzyme |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/35); total (0/35) |
| * | W3110S.gb | 4,619,283 | 0 | G | A | 100.0%
| 53.9
/ NA
| 35 | intergenic (+341/+79) | yjjV/yjjW | predicted DNase/predicted pyruvate formate lyase activating enzyme |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/35); total (0/35) |
GCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTA > W3110S.gb/4619264‑4619325
||
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGGCGC‑TGACGCGTCTTATCAGGCCTa < 1:260703/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:361157/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:951882/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:864908/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:822128/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:736292/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:727857/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:72439/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:695668/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:65949/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:612497/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:591230/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:414691/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1027163/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:223111/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1375147/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1054105/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1081937/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1109311/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1115871/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1118325/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1247639/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1393142/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1438040/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1444052/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:1461220/61‑1 (MQ=11)
gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:156019/61‑1 (MQ=11)
ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:612440/60‑1 (MQ=11)
ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:637989/60‑1 (MQ=11)
ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:186640/60‑1 (MQ=11)
ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:11452/60‑1 (MQ=11)
ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:578777/60‑1 (MQ=11)
ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:494746/60‑1 (MQ=11)
ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:82092/60‑1 (MQ=11)
cgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa < 1:930874/59‑1 (MQ=11)
||
GCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTA > W3110S.gb/4619264‑4619325
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
