Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
990,911 |
T→A |
intergenic (‑133/‑133) |
pncB ← / → pepN |
nicotinate phosphoribosyltransferase/aminopeptidase N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 990,911 | 0 | T | A | 100.0%
| 53.6
/ NA
| 32 | intergenic (‑133/‑133) | pncB/pepN | nicotinate phosphoribosyltransferase/aminopeptidase N |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (32/0); total (32/0) |
CATTACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGT > W3110S.gb/990908‑990949
|
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:15172/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:86355/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:84229/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:77528/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:76598/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:73437/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:72511/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:69063/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:68678/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:68060/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:66746/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:65086/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:62861/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:62281/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:54556/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:51211/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:4720/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:40372/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:3873/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:3820/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:34930/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:25778/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:24440/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:23548/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:21911/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:21868/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:21814/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:198/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:18038/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:17356/1‑42 (MQ=255)
cATAACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGt > 1:12887/1‑42 (MQ=255)
cATAACGCGGTGTAAGTTATAAAAATGTAGCAATaaa > 1:48762/1‑37 (MQ=38)
|
CATTACGCGGTGTATGTTATAAAAATGTAGCAATAAAGGCGT > W3110S.gb/990908‑990949
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
