Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,463,297 |
T→C |
T437A (ACG→GCG) |
yfcX ← |
fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,463,297 | 0 | T | C | 100.0%
| 42.5
/ NA
| 25 | T437A (ACG→GCG) | yfcX | fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (0/25); total (0/25) |
TTTTTCCACCGGACTGAAGAAATGCAGGCCGATAACTTGCTCAGGTCGCGTGGCGTGAGCGG > W3110S.gb/2463247‑2463308
|
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:55198/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:9186/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:87463/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:84461/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:77877/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:75952/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:71261/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:69897/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:66612/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:55590/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:55344/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:15486/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:53387/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:4688/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:4494/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:30970/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:27122/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:26837/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:25230/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:16149/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:16035/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGGGAgcgg < 1:1657/62‑1 (MQ=255)
tttttCCACCGGACTGAAGAAATGCAGGCAAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:68679/62‑1 (MQ=255)
ttttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:20482/61‑1 (MQ=255)
tttCCACCGGACTGAAGAAATGCAGGCCAATAACTTGCTCAGGTCGCGCGGCGTGAgcgg < 1:59630/60‑1 (MQ=255)
|
TTTTTCCACCGGACTGAAGAAATGCAGGCCGATAACTTGCTCAGGTCGCGTGGCGTGAGCGG > W3110S.gb/2463247‑2463308
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
