Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,592,650 |
A→G |
D622D (GAT→GAC) |
ypfI ← |
predicted hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,592,650 | 0 | A | G | 100.0%
| 34.0
/ NA
| 20 | D622D (GAT→GAC) | ypfI | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (0/20); total (0/20) |
CATAACTGCGCATCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTC > W3110S.gb/2592639‑2592700
|
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:45753/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:86120/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:73388/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:73248/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:6384/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:58815/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:58778/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:5554/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:51921/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:10749/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:44449/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:35170/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:33085/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:2865/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:24700/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:15803/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:14591/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:1169/62‑1 (MQ=255)
cATAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:11579/62‑1 (MQ=255)
aTAACTGCGCGTCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTc < 1:60878/61‑1 (MQ=255)
|
CATAACTGCGCATCACTGGCGTTTTTCTGTAAACGCCCACGCAGCGCCGGTAATGCCAGTTC > W3110S.gb/2592639‑2592700
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
