Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,866,888 A→T T174T (ACT→ACA)  nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,866,8880AT100.0% 166.9 / NA 92T174T (ACT→ACA) nlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/92);  total (0/92)

CTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCA  >  W3110S.gb/2866857‑2866916
                               |                            
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:50207/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:56600/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:56128/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:54755/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:54443/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:53596/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:53399/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:52968/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:52569/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:52129/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:51700/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:50904/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:57323/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:50102/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:50009/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:47419/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:47074/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:45927/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:45861/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:45435/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:45388/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:44841/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:44477/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:72485/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:87147/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:83976/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:8053/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:80242/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:79352/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:7750/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:76670/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:75311/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:75270/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:7428/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:44374/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:71263/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:70899/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:67321/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:66830/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:64179/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:62954/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:59235/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:59011/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:57462/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:5743/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:20671/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:4428/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:29343/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:29226/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:28441/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:27026/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:25969/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:25697/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:25167/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:24010/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:23849/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:21919/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:29816/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:20183/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:19845/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:182/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:18198/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:17078/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:16764/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:16418/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:16010/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:15109/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:11949/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:30404/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:43441/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:43398/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:43135/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:42958/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:42000/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:40312/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:40300/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:38657/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:38101/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:37807/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:37650/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:37266/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:36015/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:3594/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:32769/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:31929/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:30872/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:30290/60‑1 (MQ=255)
ctgcGTCGGCCTGGGTAATGGCATTTCCGCATGTGATTGGCGTACCGGAAGCATTACCCa  <  1:13656/60‑1 (MQ=255)
 tgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:53278/59‑1 (MQ=255)
 tgcGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:12239/59‑1 (MQ=255)
   cGTCGGCCTGGGTAATGGCATTTCCGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:11298/57‑1 (MQ=255)
                   ggCATTTCGGCCTGTGATTGGCGTACCGGAAGCATTACCCa  <  1:33154/41‑1 (MQ=38)
                               |                            
CTGCGTCGGCCTGGGTAATGGCATTTCCGCCAGTGATTGGCGTACCGGAAGCATTACCCA  >  W3110S.gb/2866857‑2866916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A