Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
826 |
0.0 |
88706 |
74.7% |
66263 |
57.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,035,461 |
A→T |
G434G (GGT→GGA) |
recJ ← |
ssDNA exonuclease, 5' ‑‑> 3'‑specific |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,035,461 | 0 | A | T | 95.7%
| 76.7
/ ‑3.8
| 46 | G434G (GGT→GGA) | recJ | ssDNA exonuclease, 5' ‑‑> 3'‑specific |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (0/44); total (0/46) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGC > W3110S.gb/3035404‑3035469
|
aaCCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACccg < 1:45682/62‑1 (MQ=255)
aCCAGTTCGCCAAACCGTTGTTGAAAGAGGTTGAATTTATCCTCTTCCAGCGACAAACccg < 1:45488/61‑1 (MQ=255)
aGTTCGCCAAAGCTTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:71122/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:64338/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:47136/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:49461/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:52705/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:53906/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:54901/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:55338/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:59781/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:60056/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:61847/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:46519/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:66779/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:67629/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:68773/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:75144/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:79044/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:79103/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:79776/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:81561/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:81876/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:30144/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:11911/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:13097/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:18141/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:18331/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:19119/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:19779/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:2086/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:22305/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:27628/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:10180/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:35288/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:37777/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:39286/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:39482/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:41195/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:41347/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:45858/62‑1 (MQ=255)
aGTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:46102/62‑1 (MQ=255)
gTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:35421/61‑1 (MQ=255)
gTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:79214/61‑1 (MQ=255)
gTTCGCCAAAGCGTTGCTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:4149/61‑1 (MQ=255)
agTTCGAATTTATCCTCTTCCAGCGACAATCCtgccgc < 1:80770/38‑1 (MQ=25)
|
AACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGC > W3110S.gb/3035404‑3035469
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
