Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,105,011 T→C F214F (TTT→TTC)  nupG → nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,105,0110TC100.0% 145.0 / NA 75F214F (TTT→TTC) nupGnucleoside transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (75/0);  total (75/0)

TGTTTAAAAACAAGCGTATGGCAATCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAAC  >  W3110S.gb/3104980‑3105039
                               |                            
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTtctc                      >  1:49543/1‑40 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAAtg                  >  1:42888/1‑44 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:63641/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:71036/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:70797/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:69772/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:69734/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:69649/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:69566/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:6741/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:65122/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:7258/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:62143/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:61973/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:5999/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:59896/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:59676/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:57426/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:56911/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:79487/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:9400/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:87960/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:86220/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:86206/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:85917/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:84058/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:83199/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:82535/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:79944/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:55147/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:7769/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:77046/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:76210/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:74849/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:74090/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:7290/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:72694/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:72641/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:18190/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:29859/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:28992/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:28243/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:23818/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:23388/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:22315/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:19108/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:18555/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:31692/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:14427/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:1419/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:14008/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:13956/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:13182/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:13151/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:12719/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:12546/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:46544/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:11350/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:54915/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:54571/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:5391/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:49968/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:49010/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:48269/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:48077/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:47724/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:55188/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:44692/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:44492/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:41171/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:40635/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:4030/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:33880/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTATGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:3177/1‑60 (MQ=255)
tGTTTAAAAACAAGCGTAAGGCAATCTTCTTCATCTTCTCAATGCTGCTGGGCGCGGAAc  >  1:78607/1‑60 (MQ=255)
                               |                            
TGTTTAAAAACAAGCGTATGGCAATCTTCTTTATCTTCTCAATGCTGCTGGGCGCGGAAC  >  W3110S.gb/3104980‑3105039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A