Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 826 0.0 88706 74.7% 66263 57.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,472,893 G→C T50T (ACC→ACG)  btuB ← vitamin B12/cobalamin outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,472,8930GC100.0% 234.3 / NA 120T50T (ACC→ACG) btuBvitamin B12/cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (120/0);  total (120/0)

Only 100 of 120 total aligned reads displayed.

GACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGGGTCAC  >  W3110S.gb/3472838‑3472898
                                                       |     
gACGTCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:53388/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGCCTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:59980/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:68064/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:69781/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:67861/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:67707/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:67121/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:66458/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:66066/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:65533/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:63586/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:6342/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:61893/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:60838/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:60768/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:56908/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:53925/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:52783/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:52714/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:52207/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:51686/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:51178/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:50749/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:50333/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:10691/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:81245/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:88355/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:87969/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:87569/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:86967/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:86546/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:85281/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:85157/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:84388/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:84320/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:82467/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:81890/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:50253/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:8096/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:80014/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:79567/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:78105/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:76424/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:75014/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:74396/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:7436/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:73427/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:73351/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:72608/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:19466/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:49480/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:26982/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:26070/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:24798/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:24607/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:24123/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:22962/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:22941/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:22803/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:2199/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:20515/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:27488/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:18486/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:17701/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:1718/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:15852/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:15752/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:15159/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:13706/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:12915/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:12817/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:12019/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:1195/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:38555/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:49164/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:48379/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:48106/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:47818/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:46008/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:45179/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:44195/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:42309/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:42009/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:39843/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:39016/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:38836/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:28351/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:38212/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:36732/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:36062/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:34759/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:34542/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:31357/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:30479/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:29851/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:2911/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTcac  >  1:28702/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTca   >  1:74593/1‑60 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGCGTaac  >  1:18709/1‑61 (MQ=255)
gACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATAGCCTGACGCGTcac  >  1:78191/1‑61 (MQ=255)
                                                       |     
GACGGCGCAGCACATCATTGACCGAGGTCGACTGCCAGCGGTCGATATCCTGACGGGTCAC  >  W3110S.gb/3472838‑3472898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A