Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,473,263 |
T→C |
intergenic (‑221/‑148) |
btuB ← / → trmA |
vitamin B12/cobalamin outer membrane transporter/tRNA (uracil‑5‑)‑methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,473,263 | 0 | T | C | 100.0%
| 45.5
/ NA
| 15 | intergenic (‑221/‑148) | btuB/trmA | vitamin B12/cobalamin outer membrane transporter/tRNA (uracil‑5‑)‑methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/0); total (15/0) |
CGCGTCAGCTCCAGATTCGCACTGGATTCCCTATTAACT > W3110S.gb/3473232‑3473270
|
cgcgTCGGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:2515625/1‑39 (MQ=38)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTaa > 1:291429/1‑37 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAAc > 1:1316287/1‑38 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:1133481/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:1881863/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:1895551/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:2028334/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:2214320/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:2470771/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:2616003/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:2846803/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:2870790/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:458757/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:854714/1‑39 (MQ=255)
cgcgTCAGCTCCAGATTCGCACTGGATTCCCCATTAACt > 1:941747/1‑39 (MQ=255)
|
CGCGTCAGCTCCAGATTCGCACTGGATTCCCTATTAACT > W3110S.gb/3473232‑3473270
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
