Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
361,710 |
C→T |
V232I (GTT→ATT) |
lacY ← |
lactose/galactose transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 361,710 | 0 | C | T | 100.0%
| 13.7
/ NA
| 6 | V232I (GTT→ATT) | lacY | lactose/galactose transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/6); total (0/6) |
AAACATCGTAGGTGCAGGAAACGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTG > W3110S.gb/361689‑361749
|
aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:1104083/61‑1 (MQ=255)
aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:582740/61‑1 (MQ=255)
aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:599935/61‑1 (MQ=255)
aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:693855/61‑1 (MQ=255)
aaaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:697093/61‑1 (MQ=255)
aaCATCGTAGGTGCAGGAAATGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTg < 1:2251712/60‑1 (MQ=255)
|
AAACATCGTAGGTGCAGGAAACGCCAATAACATACAGTGACAAAAACCACAGTTTTGGCTG > W3110S.gb/361689‑361749
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
