Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
698,544 |
A→G |
D352D (GAT→GAC) |
asnB ← |
asparagine synthetase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 698,544 | 0 | A | G | 81.2%
| 170.3
/ 30.2
| 80 | D352D (GAT→GAC) | asnB | asparagine synthetase B |
| Reads supporting (aligned to +/- strand): ref base A (8/7); new base G (65/0); total (73/7) |
| Fisher's exact test for biased strand distribution p-value = 2.03e-06 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACA > W3110S.gb/698492‑698602
|
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATACCCGCCAAACACTTCGTCAGAAcc > 1:1545580/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:262592/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2881302/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2809079/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2770956/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2687232/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2685130/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2668300/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:266222/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2904944/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2625374/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2579268/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2525486/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2512117/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1050263/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2206554/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2172565/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2151198/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:613337/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:991186/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:875215/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:850708/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:815271/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:780221/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:745453/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:688528/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:685202/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2314556/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:560174/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:518917/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:488493/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:428210/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:413028/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:37198/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:360660/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:341729/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1279343/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1538298/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1537512/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1493428/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1458504/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1424040/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:138527/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1365653/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1307368/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:211495/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1252955/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1237735/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:123421/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1163232/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1148757/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1144755/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1098349/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1710346/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1985021/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2006803/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:2069984/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1858945/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1795040/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1776271/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1560533/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1686323/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1617689/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1607720/3‑61 (MQ=255)
actCTTTAGCGTTCGGCGCTTTGTGGAAGTATAGATAACCGCCAAACACTTCGTCAGAAcc > 1:1587560/3‑61 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt > 1:485909/1‑62 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt > 1:1731010/1‑62 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt > 1:1764157/1‑62 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt > 1:1529149/1‑62 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt > 1:2781008/1‑62 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt > 1:2824189/1‑62 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAtttt > 1:2367168/1‑62 (MQ=255)
ttGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCAttt > 1:680163/1‑61 (MQ=255)
ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa < 1:565042/62‑1 (MQ=255)
ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa < 1:2132311/62‑1 (MQ=255)
ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa < 1:1093037/62‑1 (MQ=255)
ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa < 1:749506/62‑1 (MQ=255)
ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa < 1:723948/62‑1 (MQ=255)
ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa < 1:2817672/62‑1 (MQ=255)
ttCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCCTGATCTTACGCGACa < 1:723112/62‑1 (MQ=255)
tcatcaGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACa < 1:1552178/61‑1 (MQ=255)
|
GTTCTTTGGCATTCGGTGCTTTGTGGAAGTAAAGATAACCGCCGAACACTTCATCAGAACCTTCACCGGACAGCACCATTTTAATGCCCATCGCCTTGATCTTACGCGACA > W3110S.gb/698492‑698602
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
