Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
746,776 |
G→A |
V140V (GTG→GTA) |
nei → |
endonuclease VIII |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 746,776 | 0 | G | A | 83.1%
| 167.6
/ 24.4
| 77 | V140V (GTG→GTA) | nei | endonuclease VIII |
| Reads supporting (aligned to +/- strand): ref base G (0/13); new base A (64/0); total (64/13) |
| Fisher's exact test for biased strand distribution p-value = 5.44e-15 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTA > W3110S.gb/746754‑746827
|
cGAATCTGACGCCGGAGGTGGTAAAGGATCGATTATTGTCGCCGCGCTTTCGTa > 1:2284243/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTcg > 1:2583841/1‑41 (MQ=38)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt > 1:1027565/1‑53 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt > 1:1201929/1‑53 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt > 1:1474505/1‑53 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGt > 1:1759269/1‑53 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2392078/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2749300/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2691015/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:255019/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2536636/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2534779/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2507642/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:387786/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2367107/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2320981/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2287155/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2274287/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1018640/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:215862/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:555866/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:993495/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:858576/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:850466/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:7630/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:739691/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:675410/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:658259/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:618256/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2898343/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:548803/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:505014/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:498459/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:425353/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:422087/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:218222/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:360710/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1370777/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1665731/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1595637/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1576161/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1528948/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:151533/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1490190/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1436672/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1379686/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1577985/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1347056/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1338258/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1321508/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1217147/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1073188/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1070046/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1027349/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1867435/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2110173/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:2106980/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:195491/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1885007/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:187130/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1765528/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1714964/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGCGCTTTCGTa > 1:1686981/1‑54 (MQ=255)
cGAATCTGACGCCGGAGGTGGTAAAGGAACGATTATTGTCGCCGAGCTTTCGTa > 1:1207253/1‑54 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:1725238/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:1797974/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:26245/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:1561632/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:2324225/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:671675/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:2581452/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:2156996/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:821933/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:1972550/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:874918/62‑1 (MQ=255)
cGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:2735945/62‑1 (MQ=255)
ggAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTa < 1:2440522/61‑1 (MQ=255)
|
CGAATCTGACGCCGGAGGTGGTGAAAGAACGATTATTGTCGCCGCGCTTTCGTAACCGTCAGTTTGCTGGATTA > W3110S.gb/746754‑746827
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
