Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,020,915 |
+A |
coding (427/510 nt) |
sulA ← |
SOS cell division inhibitor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,020,912 | 1 | . | A | 100.0%
| 28.1
/ NA
| 10 | I144F (ATT→TTT) | sulA | SOS cell division inhibitor |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/10); total (0/10) |
AGAGGATGCGCTTACCGGACGCATAAT‑AAACCCCATAGCGTTACCTTCATTTGCCG > W3110S.gb/1020886‑1020941
|
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:1055378/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:1485598/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:1504733/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:158305/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:2220826/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:2280881/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:466308/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:684135/57‑1 (MQ=255)
agagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:686854/57‑1 (MQ=255)
gagGATGCGCTTACCGGACGCATAATAAAACCCCATAGCGTTACCTTCATTTGCCg < 1:1940000/56‑1 (MQ=255)
ataataAAACCCCATTCCGTTACCTTCATTTGCCg < 1:2315346/35‑1 (MQ=12)
|
AGAGGATGCGCTTACCGGACGCATAAT‑AAACCCCATAGCGTTACCTTCATTTGCCG > W3110S.gb/1020886‑1020941
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
