Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,428,979 |
C→T |
T271I (ACC→ATC) |
ynaA → |
predicted tail protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,428,979 | 0 | C | T | 100.0%
| 13.7
/ NA
| 6 | T271I (ACC→ATC) | ynaA | predicted tail protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/6); total (0/6) |
GAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAACCGGAATGTCGGCAAAACAAACGG > W3110S.gb/1428941‑1429002
|
gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg < 1:1584732/62‑1 (MQ=255)
gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg < 1:2075996/62‑1 (MQ=255)
gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg < 1:2259549/62‑1 (MQ=255)
gAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg < 1:2350924/62‑1 (MQ=255)
aGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg < 1:1990112/61‑1 (MQ=255)
gTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAATCGGAATGTCGGCAAAACAAACgg < 1:990624/60‑1 (MQ=255)
|
GAGTGAGCTTGAGGTTACTCAATCACAATTAAGCAAAACCGGAATGTCGGCAAAACAAACGG > W3110S.gb/1428941‑1429002
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
