Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,778,380 |
G→A |
T360T (ACG→ACA) |
ydiF → |
fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,778,380 | 0 | G | A | 100.0%
| 11.6
/ NA
| 6 | T360T (ACG→ACA) | ydiF | fused predicted acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit and beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (6/0); total (6/0) |
CCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTT > W3110S.gb/1778359‑1778432
|
ccGTGCCATTCTGGATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGAtgttt > 1:2487283/1‑62 (MQ=255)
ggctatGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt > 1:1647162/4‑62 (MQ=255)
ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt > 1:1200694/1‑62 (MQ=255)
ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt > 1:1375481/1‑62 (MQ=255)
ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt > 1:2811795/1‑62 (MQ=255)
ggATATGACATCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGtt > 1:2869852/1‑62 (MQ=255)
|
CCGTGCCATTCTGGATATGACGTCCCAGTTTGATTTTTATCACGGTGGCGGTCTGGATGTTTGTTATTTGAGTT > W3110S.gb/1778359‑1778432
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
