Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,962,789 |
C→T |
D338D (GAC→GAT) |
argS → |
arginyl‑tRNA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,962,789 | 0 | C | T | 83.5%
| 202.9
/ 29.3
| 91 | D338D (GAC→GAT) | argS | arginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (14/1); new base T (0/76); total (14/77) |
| Fisher's exact test for biased strand distribution p-value = 1.40e-15 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTC > W3110S.gb/1962737‑1962831
|
cGAAATATCGTTATGAAAGACTGCATGCCGATCGCGTGCGGTATTACATCGATTCCCGTc < 1:2421918/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGTCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1444731/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2496993/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2693975/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2691026/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2674552/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2645238/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2577692/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:255284/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:252833/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2500707/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2725911/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:549017/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2417710/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:238364/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2347966/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:635018/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2302229/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2239175/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:22274/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:324489/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:491027/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:478058/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:447480/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:438031/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:419672/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:492125/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:342990/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:342934/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:220121/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2915345/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2914546/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2895208/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:289050/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2836223/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:540660/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2793952/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2774941/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1349245/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1562443/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:839635/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:841030/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1513889/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1486164/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1440107/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1421425/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1390926/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1701208/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1330574/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1316552/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1297988/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:868132/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1244190/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1181558/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:908169/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1074259/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1851049/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2056492/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2047721/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:2041071/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:672186/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:695499/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1967127/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1894005/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1869717/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1831920/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:724315/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1803842/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:778277/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1735537/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1721462/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1705998/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1702746/60‑1 (MQ=255)
cGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTc < 1:921662/60‑1 (MQ=255)
gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:1746824/59‑1 (MQ=255)
tCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:386017/53‑1 (MQ=255)
cTGCATGCCGATCGCGTGCTGTATTACATCGATTCCCGTc < 1:115882/40‑1 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCa > 1:1969903/1‑45 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATTGGCGATCGTc > 1:1124700/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGt > 1:801186/1‑60 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:2023878/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:2323872/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:722983/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:1807355/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:2446649/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:1560152/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:15400/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:1265162/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:896993/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:2814340/1‑61 (MQ=255)
cgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTc > 1:486951/1‑61 (MQ=255)
|
CGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTC > W3110S.gb/1962737‑1962831
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
