Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,066,123 |
T→C |
Q161R (CAG→CGG) |
cobT ← |
nicotinate‑nucleotide dimethylbenzimidazole‑P phophoribosyl transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,066,123 | 0 | T | C | 90.5%
| 234.1
/ 11.0
| 85 | Q161R (CAG→CGG) | cobT | nicotinate‑nucleotide dimethylbenzimidazole‑P phophoribosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/8); new base C (76/0); total (77/8) |
| Fisher's exact test for biased strand distribution p-value = 2.29e-11 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 |
TTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGTAT > W3110S.gb/2066066‑2066129
|
tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:1493386/62‑1 (MQ=255)
tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:356063/62‑1 (MQ=255)
tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:2043560/62‑1 (MQ=255)
tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:2700160/62‑1 (MQ=255)
tttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:1424854/62‑1 (MQ=255)
tttGCCATCCCCAGTTCACCTACACCAAACAGCGGGACACCGTTTTTTGCCAGCTCCTGCGt < 1:2735413/62‑1 (MQ=255)
ttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:135978/61‑1 (MQ=255)
ttGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGt < 1:1481553/61‑1 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2491921/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2490938/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:333483/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:317495/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2876933/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2851619/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2851025/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2748318/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2736240/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2670099/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2616627/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2717693/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2561215/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:490880/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2675470/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2623759/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2575207/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:634888/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:961039/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:881997/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:796818/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:758441/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:735047/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:727396/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:72227/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:719411/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:637674/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:37825/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:628966/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:625307/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:604252/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:596199/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:584555/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:574116/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:521995/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2310922/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:463631/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1602985/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1755908/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2388318/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1749053/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1744406/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1732899/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1724385/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1650646/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:161596/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1603605/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1752952/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1598590/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:150839/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:141634/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1408176/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1215083/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1094202/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1091027/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1087969/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2084980/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2340050/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2317011/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1026078/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2302448/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2295774/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2266650/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2214773/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1818521/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2074901/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2048949/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2025625/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1965940/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1962917/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1877108/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1870377/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCCGCGtat > 1:1845359/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGCCAGCTCCAGCGtat > 1:1741235/1‑36 (MQ=255)
aCAGCGTGACACCGTTTTTTGACAGCTCCCGCGtat > 1:120643/1‑36 (MQ=37)
aCAGCGTGACACCGTTTTATGCCAGCTCCCGCGtat > 1:1486192/1‑36 (MQ=37)
aCAGCGTCACACCGTTTTTTGCCAGCTCCCGCGtat > 1:2307615/1‑36 (MQ=37)
|
TTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTGCCAGCTCCTGCGTAT > W3110S.gb/2066066‑2066129
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
