Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,383,383 |
G→T |
L183I (CTT→ATT) |
menD ← |
bifunctional 2‑oxoglutarate decarboxylase and SHCHC synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,383,383 | 0 | G | T | 100.0%
| 94.4
/ NA
| 29 | L183I (CTT→ATT) | menD | bifunctional 2‑oxoglutarate decarboxylase and SHCHC synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (29/0); total (29/0) |
TCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGC > W3110S.gb/2383343‑2383403
|
tcgtcCTGCCCCCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTc > 1:1497267/1‑50 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGt > 1:1435039/1‑52 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2571730/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:90089/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:419316/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:35053/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:345120/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:303044/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:300350/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:294838/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2850147/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2727559/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2685855/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2684575/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2665500/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2658310/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2657939/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1062728/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2195926/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2177966/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:2165413/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1912598/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1833198/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1753742/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1485187/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1477579/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1190290/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1169846/1‑61 (MQ=255)
tcgtcCTGCCACCAGTCGCCCAGCCGCTGTTGCCAGCTAATCCCGGTGTCGTCCATTTCGc > 1:1156097/1‑61 (MQ=255)
|
TCGTCCTGCCACCAGTCACCCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGC > W3110S.gb/2383343‑2383403
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
