Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,607,203 |
T→A |
F21I (TTC→ATC) |
hyfG → |
hydrogenase 4, subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,607,203 | 0 | T | A | 100.0%
| 19.3
/ NA
| 9 | F21I (TTC→ATC) | hyfG | hydrogenase 4, subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (9/0); total (9/0) |
CGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTG > W3110S.gb/2607159‑2607220
|
cGTCAAATCGTGTCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:2659937/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:155641/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:1574745/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:1639760/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:165685/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:2026694/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:2702160/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:581887/1‑62 (MQ=255)
cGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGATCCCCGGCGCGGTGCTg > 1:677285/1‑62 (MQ=255)
|
CGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTG > W3110S.gb/2607159‑2607220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
