Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,682,030 |
Δ2 bp |
coding (512‑513/1194 nt) |
yphH → |
predicted DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,682,029 | 0 | G | . | 100.0%
| 29.3
/ NA
| 8 | coding (511/1194 nt) | yphH | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (8/0); total (8/0) |
| * | W3110S.gb | 2,682,030 | 0 | A | . | 100.0%
| 29.3
/ NA
| 8 | coding (512/1194 nt) | yphH | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (8/0); total (8/0) |
GTGACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTGGAGGTGAAATATC > W3110S.gb/2681978‑2682041
||
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAatat > 1:2549305/1‑61 (MQ=255)
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAatat > 1:2886660/1‑61 (MQ=255)
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAatat > 1:958036/1‑61 (MQ=255)
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc > 1:1453928/1‑62 (MQ=255)
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc > 1:1661006/1‑62 (MQ=255)
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc > 1:181344/1‑62 (MQ=255)
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc > 1:2272169/1‑62 (MQ=255)
gtgACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTG‑‑GGTGAAATATc > 1:509444/1‑62 (MQ=255)
||
GTGACCGGCGTGTCGCAAACCATGCCGCAAGCGCCGTGGACAACGCCGGTGGAGGTGAAATATC > W3110S.gb/2681978‑2682041
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
