Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,692,685 |
A→T |
A505A (GCT→GCA) |
purL ← |
phosphoribosylformyl‑glycineamide synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,692,685 | 0 | A | T | 88.5%
| 145.5
/ 9.9
| 52 | A505A (GCT→GCA) | purL | phosphoribosylformyl‑glycineamide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base T (46/0); total (52/0) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 |
AGTTCCGGCATGGCGTTAGAAAGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCA > W3110S.gb/2692656‑2692735
|
aGTTCCGGCATGGCGTTAGAAAGCCCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1903375/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAgg > 1:1922911/1‑54 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTgggct > 1:1614457/1‑60 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2848161/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2354677/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1054916/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2367708/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:25649/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2642048/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2648651/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2711485/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2717419/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2722426/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2741233/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2360379/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2877896/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:312167/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:325445/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:451477/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:666870/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:674976/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:714743/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:85182/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:906975/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:978034/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2323539/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1156104/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1289461/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1346068/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1364267/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1507094/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1543380/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1654461/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:180389/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:18251/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:1910692/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2035160/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2328783/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2307826/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2066998/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGtt > 1:2282376/1‑62 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:1678703/1‑61 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:551184/1‑61 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:675764/1‑61 (MQ=255)
aGTTCCGGCATGGCGTTAGAAAGACCGCCTGCGCCAACGTCGTGGATAAACAGGATTGGGt > 1:1304923/1‑61 (MQ=255)
aGTTCCGGCATGGCGTTAGAAACACCGCCTGCGCCAACGTCGTGTATAAACAGGAt > 1:636337/1‑56 (MQ=255)
aaaGCCCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGcc > 1:2700543/1‑60 (MQ=255)
aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTg > 1:2834512/1‑39 (MQ=255)
aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:1089947/1‑61 (MQ=255)
aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:51937/1‑61 (MQ=255)
aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:1456159/1‑61 (MQ=255)
aaaGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCa > 1:1719097/1‑61 (MQ=255)
|
AGTTCCGGCATGGCGTTAGAAAGACCGCCAGCGCCAACGTCGTGGATAAACAGGATTGGGTTGGCATCACCAAGCTGCCA > W3110S.gb/2692656‑2692735
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
