Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,783,860 |
(A)8→7 |
intergenic (‑193/+558) |
ypjC ← / ← ileY |
hypothetical protein/tRNA‑Ile |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,783,853 | 0 | A | . | 100.0%
| 15.9
/ NA
| 5 | intergenic (‑186/+565) | ypjC/ileY | hypothetical protein/tRNA‑Ile |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (3/2); total (3/2) |
TATTTCGCTGTATGATAAAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATT > W3110S.gb/2783837‑2783906
|
tATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGtt < 1:665854/62‑1 (MQ=255)
tATTTCGCTGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGtt < 1:830357/62‑1 (MQ=255)
tGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAAtt > 1:1027044/1‑61 (MQ=255)
tGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAAtt > 1:2301101/1‑61 (MQ=255)
tGTATGAT‑AAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAAtt > 1:432269/1‑61 (MQ=255)
|
TATTTCGCTGTATGATAAAAAAAACCGTTATAATTTATTAGTGAAAATCGTTTTTCAAGTGTTAGAAATT > W3110S.gb/2783837‑2783906
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
