Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,837,372 |
+A |
coding (552/1014 nt) |
ascG ← |
DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,837,369 | 1 | . | A | 100.0%
| 14.1
/ NA
| 6 | F185F (TTC→TTT) | ascG | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (0/6); total (0/6) |
CTGGTGGGGGAATCCATCGAGCCGGTAAGG‑AAAGCAATCTCCTGATGCCCGGCGTTTATCA > W3110S.gb/2837340‑2837400
|
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTtcatca < 1:1380689/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1015995/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1215628/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1512639/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:1889357/62‑1 (MQ=255)
cTGGTGGGGGAATCCATCGAGCCGGTAAGGAAAAGCAATCTCCTGATGCCCGGCGTTTATCa < 1:341162/62‑1 (MQ=255)
|
CTGGTGGGGGAATCCATCGAGCCGGTAAGG‑AAAGCAATCTCCTGATGCCCGGCGTTTATCA > W3110S.gb/2837340‑2837400
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
