Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R1 375 42.1 2927644 71.4% 2090337 57.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,247,795 C→T V57V (GTC→GTT)  yqjC → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,247,7950CT100.0% 200.2 / NA 61V57V (GTC→GTT) yqjCconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/24);  total (37/24)

GCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAG  >  W3110S.gb/3247736‑3247852
                                                           |                                                         
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2843543/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1652281/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1682733/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1686009/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2003777/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2081837/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2154092/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2301396/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2490040/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2689713/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:11092/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2875641/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:2910417/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:570415/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:60250/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:640929/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:727079/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:757628/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:773294/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:927710/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1615364/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:113258/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1417623/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1597766/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1398197/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1254500/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1265650/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1289870/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1396285/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1483512/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1456696/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1367855/1‑62 (MQ=255)
gCCGAAAAACACCAAAACCAGAATCGTATTGAAGGTCTGAATAAAGCCCTGAGTGAAGTTCg                                                         >  1:1312438/1‑62 (MQ=255)
    aaaaaCACCAAAACC‑‑AATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:404487/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2909275/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2887569/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2597048/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1138744/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:768789/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1045937/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1658866/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1449083/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2265149/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2531778/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:217122/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1809637/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1530173/62‑1 (MQ=255)
      aaaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGCGAAGTTCGGGCCaa                                                   <  1:1855105/62‑1 (MQ=255)
       aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1386969/61‑1 (MQ=255)
       aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:160745/61‑1 (MQ=255)
       aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:455970/61‑1 (MQ=255)
       aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:1895426/61‑1 (MQ=255)
       aaCACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:72259/61‑1 (MQ=255)
            cAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTTCGGGCCaa                                                   <  1:2216360/56‑1 (MQ=255)
                                           aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCagaa               >  1:2676262/1‑61 (MQ=255)
                                           aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCagaa               >  1:1569684/1‑61 (MQ=255)
                                           aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCagaa               >  1:2676335/1‑61 (MQ=255)
                                           aaGCCCTGAGTGAAGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCaga                >  1:201115/1‑60 (MQ=255)
                                                       aaGTTCGGGGCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAg  <  1:164881/62‑1 (MQ=255)
                                                       aaGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAg  <  1:410087/62‑1 (MQ=255)
                                                       aaGTTCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAg  <  1:2235846/62‑1 (MQ=255)
                                                           |                                                         
GCCGAAAAACACCAAAACCAGAATCGTATTGACGGTCTGAATAAAGCCCTGAGTGAAGTCCGGGCCAACTGTTCAGATAGCCAGCTGCGTGCCGATCATCAGAAGAAAATCGCAAAG  >  W3110S.gb/3247736‑3247852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A