Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,256,157 |
G→A |
S149S (TCC→TCT) |
yhaO ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,256,157 | 0 | G | A | 100.0%
| 26.4
/ NA
| 10 | S149S (TCC→TCT) | yhaO | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/0); total (10/0) |
AATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAT > W3110S.gb/3256134‑3256194
|
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGa > 1:638573/1‑57 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACGAt > 1:2865304/1‑61 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCa > 1:2349585/1‑60 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt > 1:1000211/1‑61 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt > 1:1702381/1‑61 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt > 1:1807526/1‑61 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt > 1:1876324/1‑61 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt > 1:260808/1‑61 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAt > 1:787757/1‑61 (MQ=255)
aaTAACAATTTCTCGCCGCGTGAAGAGATCGCCACCAGAATGCAAATCAGTACCAGACAAt > 1:66384/1‑61 (MQ=255)
|
AATAACAATTTCTCGCCGCGTGAGGAGATCGCCACCAGAATGCAAATCAGTACCAGACCAT > W3110S.gb/3256134‑3256194
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
