Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,393,749 |
T→C |
I789V (ATA→GTA) |
yhdP ← |
conserved membrane protein, predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,393,749 | 0 | T | C | 92.0%
| 254.1
/ 7.8
| 88 | I789V (ATA→GTA) | yhdP | conserved membrane protein, predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base C (81/0); total (81/7) |
| Fisher's exact test for biased strand distribution p-value = 1.58e-10 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 |
Only 100 of 131 total aligned reads displayed.
CACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCAC > W3110S.gb/3393720‑3393804
|
cacaTTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGAt < 1:1650359/62‑1 (MQ=255)
cacaTTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGAt < 1:271645/62‑1 (MQ=255)
cacaTTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGAt < 1:2847214/62‑1 (MQ=255)
cTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGc < 1:563448/62‑1 (MQ=255)
gCTCTACGTTATACGTCGCGCCATCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1041664/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTc > 1:198018/1‑40 (MQ=25)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAAt > 1:1029502/1‑43 (MQ=25)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACtt > 1:1445926/1‑51 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTa > 1:722558/1‑53 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCa > 1:1032896/1‑56 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:183128/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:61612/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1811851/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1971465/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1775687/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:333891/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:608667/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2020781/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:605732/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2081421/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2126176/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:217684/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2202408/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:226625/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:469343/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2439102/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2510815/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:376455/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2593984/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2821741/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:282357/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:286207/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:2893872/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:308891/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:310133/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1765489/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1341524/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1237711/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1356169/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:835631/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1229992/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1166093/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:842949/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:139024/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:909231/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:953260/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:697822/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1018386/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1584379/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1592047/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:657771/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1624598/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1627514/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1658158/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1664774/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1672493/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1676764/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1753348/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:647965/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCcca > 1:2046443/1‑61 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCACTTTACCATCcca > 1:1775975/1‑61 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCCAC‑TTACCATCccac > 1:1287163/1‑61 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGCATGATAAGGCAGCTCAATGCCAACTTTACCATCccac > 1:635494/1‑62 (MQ=255)
gCTCTACGTTATACGTCGCGCCAGAATGATAAGGCAGCTCAATGCCCACTTTACCATCccac > 1:1182128/1‑62 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:365734/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:335481/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:393922/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:422478/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:434951/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:816932/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:477511/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:711272/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:658747/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:953660/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1773387/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:109062/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:109670/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1121555/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1149725/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1358529/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1360518/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1474687/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1528821/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1533719/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1547304/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:160033/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1765336/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:329642/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:1887930/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2015020/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2067588/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2136798/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2209190/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2395574/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2426917/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2436845/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2523574/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2576249/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2674506/38‑1 (MQ=255)
tGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATg < 1:2695487/38‑1 (MQ=255)
|
CACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCAC > W3110S.gb/3393720‑3393804
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
