Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I3 R1 375 42.1 2927644 71.4% 2090337 57.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,578,721 T→C T25T (ACT→ACC)  glnA → glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,578,7210TC100.0% 249.1 / NA 73T25T (ACT→ACC) glnAglutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (73/0);  total (73/0)

CTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACTAAAGGTAAAGAAC  >  W3110S.gb/3578674‑3578734
                                               |             
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCTCCGATACCAAAGGTAAAGAAc  >  1:1378278/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2650812/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2828768/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2810115/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2789590/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2734661/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2727630/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2713592/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2675328/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2662721/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2871830/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2558034/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2509704/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2504593/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2436146/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2429861/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2413508/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2258827/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2244952/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:687185/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:87026/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:843598/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:836991/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:794775/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:793077/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:759918/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:758102/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:743044/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2231222/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:64720/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:52313/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:489433/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:378160/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:366083/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:356607/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2879441/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1197605/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1020185/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1462328/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1427515/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1344783/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1319498/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1261414/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1249688/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1221763/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1465893/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1116362/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1096793/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1092233/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1083131/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1059323/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1049182/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1047475/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1031583/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1795709/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2162654/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2148276/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:2007956/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1971871/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1966419/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1961300/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1950225/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:192537/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1854492/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:150045/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1790625/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1691546/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1644173/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1623925/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1614892/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACCAAAGGTAAAGAAc  >  1:1581744/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACAGATACCAAAGGTAAAGAAc  >  1:450645/1‑61 (MQ=255)
cTGAACGAGCACGAAGTGAAGTTTGTTGATTAGCGCTTCACCGATACCAAAGGTAAAg     >  1:303259/1‑58 (MQ=255)
                                               |             
CTGAACGAGCACGAAGTGAAGTTTGTTGATTTGCGCTTCACCGATACTAAAGGTAAAGAAC  >  W3110S.gb/3578674‑3578734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A