Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,669,364 |
+A |
coding (901/1260 nt) |
rho ← |
transcription termination factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,669,361 | 1 | . | A | 100.0%
| 56.6
/ NA
| 19 | G302C (GGT→TGT) | rho | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (19/0); total (19/0) |
TGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACC‑AAAGAAGCGTTTCGGACGA > W3110S.gb/3669322‑3669380
|
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:2347874/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:975547/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:952763/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:783658/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:724386/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:492733/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:43684/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:2527356/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:2494121/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:1009304/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:2241544/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:201544/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:1673293/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:1663449/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:1649667/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:1565771/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:1561367/1‑60 (MQ=255)
tGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:1319440/1‑60 (MQ=255)
tGGTAAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACCAAAAGAAGCGTTTCGGACGa > 1:732991/1‑60 (MQ=255)
|
TGGTCAGGCTGCCGCCCTCTTCCACGTTACGCGCCGCACC‑AAAGAAGCGTTTCGGACGA > W3110S.gb/3669322‑3669380
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
