Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,675,341 |
T→A |
S222C (AGC→TGC) |
rep ← |
DNA helicase and single‑stranded DNA‑dependent ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,675,341 | 0 | T | A | 100.0%
| 43.1
/ NA
| 20 | S222C (AGC→TGC) | rep | DNA helicase and single‑stranded DNA‑dependent ATPase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (20/0); total (20/0) |
CCAGCAGTTTCACCAGCTCATACTGGCTGGTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAGCG > W3110S.gb/3675314‑3675398
|
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2898602/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2275134/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2349785/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2151663/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2097116/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2438582/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1999855/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2921049/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1754213/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1730205/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1720946/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1630491/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1480185/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:2791/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:73529/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1264389/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:745508/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:1227808/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:77495/1‑42 (MQ=19)
ccaGCAGCTTGACCAGCTCGTACTGGCAGGTATTGGTGTCCt > 1:900961/1‑42 (MQ=19)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATATCGAATTTTGTTCTGCCAgcg < 1:402653/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2734867/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2849681/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:948802/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:326880/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:350166/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:352589/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:424683/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:494499/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:70896/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1166724/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1818466/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1184240/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1184950/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1220967/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1242758/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1353351/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1363022/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1587326/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1707098/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2690957/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:1865536/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2027867/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:220910/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2487186/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2510928/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2582557/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2626194/61‑1 (MQ=255)
ggctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:2663636/61‑1 (MQ=255)
gctggTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAgcg < 1:710750/60‑1 (MQ=255)
|
CCAGCAGTTTCACCAGCTCATACTGGCTGGTGTTGGTGTCCTGATACTCATCCACCAGCAGATAGCGAATTTTGTTCTGCCAGCG > W3110S.gb/3675314‑3675398
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
