Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,683,940 |
C→T |
V86V (GTG→GTA) |
ilvE ← |
branched‑chain amino‑acid aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,683,940 | 0 | C | T | 100.0%
| 152.8
/ NA
| 47 | V86V (GTG→GTA) | ilvE | branched‑chain amino‑acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/47); total (0/47) |
CGACGAAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATCACGTCACGACAAGCTTCCATCAGCTCATCAATGCT > W3110S.gb/3683888‑3683975
|
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:167209/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:45854/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:507467/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:1910585/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:706394/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:1907584/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:355462/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:204247/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:2154468/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:2280386/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:2768175/61‑1 (MQ=255)
cgacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:106803/61‑1 (MQ=255)
gacgaAGATCAGCGGACGGATATAGGCGCTGGTTAGATTGTTTTTGCGGAttacgtcacg < 1:1191614/60‑1 (MQ=255)
gacgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:1220794/60‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:2718925/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:2107328/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:29173/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:2359483/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:434274/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:2764367/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:2678611/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:2706190/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1877114/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1824109/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1756743/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1702714/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1685051/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1363037/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:710186/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:754419/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:774999/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1117628/62‑1 (MQ=255)
acgaAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACa < 1:1005000/62‑1 (MQ=255)
cAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGAttacgtcacg < 1:2843831/51‑1 (MQ=255)
cggacggaTATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCa < 1:2196072/62‑1 (MQ=255)
cggaTATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCtc < 1:932494/62‑1 (MQ=255)
cggaTATAGGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCtc < 1:1045016/62‑1 (MQ=255)
cggaTATACGCGCTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCtc < 1:337436/62‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1943443/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:94103/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1083396/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:928589/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:918543/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1199653/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1257558/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1908486/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1930577/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:48082/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:48013/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:2638656/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1970445/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:363186/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:33515/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:329500/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:2065486/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:2881777/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:2166465/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:2791127/61‑1 (MQ=255)
cgcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:2540142/61‑1 (MQ=255)
gcTGGTGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1160564/60‑1 (MQ=255)
tGAGATTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:620999/55‑1 (MQ=255)
aTTGTTTTTGCGGATTACGTCACGACAAGCTTCCATCAGCTCATCAATGct < 1:1985203/51‑1 (MQ=255)
|
CGACGAAGATCAGCGGACGGATATAGGCGCTGGTGAGATTGTTTTTGCGGATCACGTCACGACAAGCTTCCATCAGCTCATCAATGCT > W3110S.gb/3683888‑3683975
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
