Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,726,860 |
G→A |
V192V (GTG→GTA) |
pstC → |
phosphate transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,726,860 | 0 | G | A | 75.4%
| 100.4
/ 33.6
| 61 | V192V (GTG→GTA) | pstC | phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/15); new base A (46/0); total (46/15) |
| Fisher's exact test for biased strand distribution p-value = 1.42e-14 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 |
TCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTG > W3110S.gb/3726814‑3726919
|
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAAcccc > 1:467617/1‑61 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2792104/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1833312/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:354675/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1924410/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:332819/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:314329/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2867021/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2854933/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2132095/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2193854/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2216131/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1829973/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2787044/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2236491/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2602307/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2268969/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2554861/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2398544/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2403897/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:254491/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2480572/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:2539267/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1488706/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:903372/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:887152/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:840822/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1197247/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:83259/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1332728/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1339519/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1365881/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1397824/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1452019/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:14735/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:508960/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1571038/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1584321/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:481196/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1710830/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:464419/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1767730/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:1776457/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAACAAACCCCg > 1:38030/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATTCGAAAAAACCCCg > 1:1914358/1‑62 (MQ=255)
tCGCCATCATGATTATTCCGTACATTGCAGCGGTAATGCGCGATGTATACGAAAAAAccc > 1:1024131/1‑60 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTcag < 1:1585438/61‑3 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:1961214/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:57615/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:1491982/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:165388/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:253329/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:409664/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:609550/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:1795220/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:124949/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:2233612/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:2259217/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:100148/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgg < 1:977952/61‑1 (MQ=255)
tACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCgc < 1:104882/61‑2 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCgg > 1:59882/1‑36 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCCCCACCt > 1:307217/1‑60 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCt > 1:1975505/1‑60 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:422435/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:751394/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:758604/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:1046567/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:579042/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:1749415/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:402154/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:1886022/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:303767/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2092590/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2229424/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2325238/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTg > 1:2450041/1‑61 (MQ=255)
tgtTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACATg > 1:211780/1‑61 (MQ=255)
|
TCGCCATCATGATTATTCCGTACATTGCGGCGGTAATGCGTGATGTGTTCGAACAAACCCCGGTGATGATGAAAGAGTCGGCCTACGGTATTGGCTGCACCACCTG > W3110S.gb/3726814‑3726919
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
