Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,964,846 |
C→A |
T262T (ACG→ACT) |
yhjE ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,964,846 | 0 | C | A | 81.6%
| 96.0
/ 22.9
| 49 | T262T (ACG→ACT) | yhjE | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/9); new base A (0/40); total (0/49) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 |
GTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAC > W3110S.gb/3964805‑3964893
|
gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:2530381/62‑1 (MQ=255)
gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:1164565/62‑1 (MQ=255)
gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:1428183/62‑1 (MQ=255)
gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:2245264/62‑1 (MQ=255)
gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:2762353/62‑1 (MQ=255)
gTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:2021432/62‑1 (MQ=255)
tACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:2759007/61‑1 (MQ=255)
tACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:2157739/61‑1 (MQ=255)
atgatgTAAAACAGCGTATAGGTTGCCAGCATAAt < 1:1510610/35‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:586037/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:246909/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2538171/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2653890/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2878423/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2889727/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2915856/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2314157/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:760254/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:791988/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:800214/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:839376/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:849790/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:86068/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:909609/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:921282/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:923190/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:964658/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2404555/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1181306/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1220395/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:128021/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1400000/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1504910/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1697816/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1732992/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1795830/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1922641/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1928434/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:1936024/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:197099/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2130291/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:218316/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2184655/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:221025/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:223671/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2285794/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2342319/62‑1 (MQ=255)
atgatgTAAAACAGAGTATAAGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:624463/62‑1 (MQ=255)
atgaagTAAAACAGAGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAc < 1:2453713/62‑1 (MQ=255)
|
GTACTAAAGGTCATAGAGTAGACCGTCATGATGTAAAACAGCGTATAGGTTGCCAGCATAATGAACGTACCCAGTACGGTTACGCGAAC > W3110S.gb/3964805‑3964893
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
