Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,178,794 |
A→T |
L53I (TTA→ATA) |
gspH ← |
predicted general secretory pathway component, cryptic |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,178,794 | 0 | A | T | 100.0%
| 41.3
/ NA
| 15 | L53I (TTA→ATA) | gspH | predicted general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (0/15); total (0/15) |
GACCACTGAGCGTGGCGCGGTCGATGGCTAATTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACATCCTC > W3110S.gb/4178764‑4178854
|
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:973046/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:428191/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:2788995/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:2734371/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:2695188/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:615140/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:2413682/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:2294163/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:2047777/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:1923308/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:1631055/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:1487782/62‑1 (MQ=255)
gACCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:1356224/62‑1 (MQ=255)
aCCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCTGTAAACCGCGCCGCTGtt < 1:2523727/61‑1 (MQ=255)
aCCACTGAGCGTGGCGCGGTCGATGGCTATTTCAAGTGCAGCGGTAAACCGCGCCGCTGtt < 1:171319/61‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:953678/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:924024/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:693301/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:963220/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:2644212/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:591127/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:510479/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:445016/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:125294/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:2331372/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:2277566/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:1938574/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:1544999/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:1262498/62‑1 (MQ=255)
aaTTCAAGTGCAGCGGTAAAACGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACAtcctc < 1:475741/62‑1 (MQ=255)
|
GACCACTGAGCGTGGCGCGGTCGATGGCTAATTCAAGTGCAGCGGTAAACCGCGCCGCTGTTTCGCGCGCACGAGTGCTGGCGACATCCTC > W3110S.gb/4178764‑4178854
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
