Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,418,573 |
C→T |
G288G (GGC→GGT) |
yjfC → |
predicted synthetase/amidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,418,573 | 0 | C | T | 100.0%
| 66.5
/ NA
| 21 | G288G (GGC→GGT) | yjfC | predicted synthetase/amidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/0); total (21/0) |
CCTGGTTCGATGGCGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATC > W3110S.gb/4418560‑4418621
|
ccTGTTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:580311/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCg > 1:2292620/1‑52 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAAt > 1:226255/1‑61 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:2416325/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:986201/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:955417/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:888186/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:775958/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:525044/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:463365/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:2874580/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:2762461/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:2714434/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:120221/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:2341217/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:2138497/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:1871733/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:1743097/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:1727775/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:1593701/1‑62 (MQ=255)
ccTGGTTCGATGGTGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATc > 1:1421097/1‑62 (MQ=255)
|
CCTGGTTCGATGGCGAGAAACCGCAGATTGCCGCTGGCGAAAGCTATGTGCGTAAACCAATC > W3110S.gb/4418560‑4418621
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
