Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I3 R1
|
375 |
42.1 |
2927644 |
71.4% |
2090337 |
57.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
4,467,333 |
G→A |
H4Y (CAT→TAT) |
nrdD ← |
anaerobic ribonucleoside‑triphosphate reductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 4,467,333 | 0 | G | A | 100.0%
| 166.2
/ NA
| 50 | H4Y (CAT→TAT) | nrdD | anaerobic ribonucleoside‑triphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/28); total (22/28) |
GCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTGG > W3110S.gb/4467275‑4467385
|
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:396367/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1876766/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:2086394/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:2308734/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:2710014/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:2762894/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:2827498/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:2881724/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:290929/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1044120/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:55882/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:599430/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:74595/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:747458/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:87587/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:951252/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:952134/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:954717/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1610646/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1029830/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1077146/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1168321/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1300187/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1494237/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1635120/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1674205/62‑1 (MQ=255)
gCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1724882/62‑1 (MQ=255)
aTTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATACgg < 1:1067697/43‑1 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGgtgt > 1:607029/1‑42 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:1511910/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:708824/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:691640/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:1934448/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:1446546/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:513938/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:1150174/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:2736689/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:2234073/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:1726174/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:2450647/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAttt > 1:1722916/1‑60 (MQ=255)
aaaCGGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATGATCGCTCCATAtt > 1:1508667/1‑59 (MQ=255)
aaaCCGCACTTTGCAGCCGTCTCGTTTCATCACATACGGTGTCATg > 1:2150902/1‑46 (MQ=255)
atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg > 1:1809788/1‑60 (MQ=255)
atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg > 1:1177998/1‑60 (MQ=255)
atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg > 1:1968664/1‑60 (MQ=255)
atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg > 1:1764600/1‑60 (MQ=255)
atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg > 1:2604117/1‑60 (MQ=255)
atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg > 1:2458022/1‑60 (MQ=255)
atcaCATACGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTgg > 1:2375190/1‑60 (MQ=255)
|
GCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGAAATTGG > W3110S.gb/4467275‑4467385
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
