Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F28 I2 R1 52 300.6 3297834 97.8% 3225281 57.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 354,373 C→G L230V (CTG→GTG)  cysS → cysteinyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE354,3730CG87.2% 257.3 / 24.9 86L230V (CTG→GTG) cysScysteinyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base G (0/75);  total (3/83)
Fisher's exact test for biased strand distribution p-value = 1.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACC  >  minE/354324‑354414
                                                 |                                         
cTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGc                               >  1:1235105/1‑62 (MQ=255)
cTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGc                               >  1:1481191/1‑62 (MQ=255)
cTGCAAGCAGCTGGGGAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGc                               >  1:2632870/1‑62 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:2701187/62‑1 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:2161134/62‑1 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:1845435/62‑1 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:429608/62‑1 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:2493552/62‑1 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:3180863/62‑1 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:2238592/62‑1 (MQ=255)
  gCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGcac                             <  1:16134/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:322196/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:369813/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:3204915/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:3204753/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:3174073/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:3172318/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:3143416/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2992110/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2953549/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2893534/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2891209/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2886417/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2866311/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:286223/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2809153/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:631336/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1106721/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:92452/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:840642/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:793084/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:77300/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:730790/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:700331/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:649263/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:634012/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:328596/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:59597/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:591959/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:527010/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:49114/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:484843/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:434459/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:38536/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:970431/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:349290/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1406364/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:275811/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:188122/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1877689/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1813723/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:17752/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1703069/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1664066/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1541086/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1431341/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1881588/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1338150/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1321822/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1316800/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1297825/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1257363/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1250466/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:124738/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1197791/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1130849/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2008143/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2742482/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2687201/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2684208/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:267303/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2649123/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2348458/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2306346/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2304759/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2242556/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2234494/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2086327/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1972700/62‑1 (MQ=255)
                             tCCACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATAGCGCAGTCCAcc  <  1:280073/62‑1 (MQ=255)
                              ccACGGCGGCTGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2184393/61‑1 (MQ=255)
                              ccACGGCGGCGGTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2116388/61‑1 (MQ=255)
                                   gcggcggTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:1786064/56‑1 (MQ=255)
                                     ggcggTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2767677/54‑1 (MQ=255)
                                      gcggTTCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:2736584/53‑1 (MQ=255)
                                           tCAGACGTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCAcc  <  1:3050808/48‑1 (MQ=255)
                                                 |                                         
CTGCAAGCAGCTGGGTAACCACTTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCGCAGTCCACC  >  minE/354324‑354414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A